Genuin Biotechnologies- One Stop Solutions for Gene Silencing

​At Genuin Biotechnologies LLC, we use our unique ShGE™ gene silencing platform to achieve high throughput, low-cost verification of antibody specificity. Our platform uses lentivirus-mediated short hairpin RNA (shRNA) to silence or knock-down (KD) the messenger RNA (mRNA) encoded by a specific gene, thereby causing the protein encoded by the gene to disappear or become significantly down-regulated. If an antibody can recognize a signal in a wild-type (WT) cell or its lysate, but the signal disappears or significantly decreases in the KD cell or its lysate, the antibody is considered to be highly specific. Based on the above principle, we verify and screen antibodies using techniques such as Western blotting (WB), immunocytochemistry (IC), and flow cytometry (FCM).

Genuin Biotechnologies’ platform is in a class by itself in the biotechnology field, and the following numbers can further illustrate the advancement of our platform technology:

1. We can perform KD on 500 genes every week;
2. The specificity of the antibodies we have verified can reach 100%;
3. We can verify and screen nearly 1,000 highly specific antibodies in a year;
4. We cover a wider range of genes than any knockout (KO) strategy. Nearly 1/4 of human genes are essential, making it impossible to use knockout (KO) technique to validate antibodies corresponding to these genes. In theory, our platform can silence or KD nearly all protein-coding genes.
5. Additionally, we can serve you with our highly specific lentiviral library generated during antibody validation. For example, if you intend to silence a specific gene that has been in our library, we can deliver the verified virus to you immediately so you can generate a KD cell line within one week. With multiple lentiviral particles, you can silence several genes simultaneously.