Frequently Asked Questions
You Asked, We Answered
What differentiates GenuIN Biotech from other competitors in the antibody market?
Our antibodies are tested using proprietary knockdown (KD) and knockout (KO) techniques. Detecting a band of an expected size in a Western blot does not always mean that the antibody is specific. Further, an antibody that works well in Western blotting does not necessarily work for immunocytochemistry or other applications. In GenuIN Biotech, each and every antibody is tested for its specificity, sensitivity, and reproducibility.
What percentage of antibodies in the market have passed your rigorous testing?
In Western blotting, if a band at the expected size can be detected by an antibody in wild-type (WT) cell lysates but disappears or significantly diminishes in KD/KO cell lysates, the antibody is considered to be specific. Similarly, if an antibody can stain the WT cells (as indicated by strong fluorescent signals), but the signals are either lost or significantly reduced in KD/KO cells, this antibody is deemed specific in immunofluorescence (IF) or flow cytometry (F) applications. Based on our criteria, an average of 30% antibodies tested are specific for use in Western blotting. When it comes to immunocytochemistry and immunofluorescence (IC-IF) or flow cytometry (F), only 20% of antibodies are specific.
How can you guarantee customer satisfaction?
Each of our antibodies is tested and labelled for use in a specific application(s). When following the application instructions and appropriate protocols for a specific antibody, you should be able to obtain the same or similar results as we have. Above all, we only sell antibodies that pass our KD/KO and application tests. If a certain antibody does not pass our testing or the results are not of publication quality, we will not sell it.
Why do you use a knockdown-knockout (KD-KO) combination strategy rather than just one technique to test our antibodies?
Some companies have begun using KO strategy to test antibodies, yet some genes are so vital to cells that knocking out these genes will cause the cells to die. In this regard, our knockdown (KD) strategy can compensate for such a shortcoming in that by lowering the protein expression through RNAi rather than completely eliminating it. The cells are not only saved from KO lethality, but also the specificity of an antibody in these cells can be tested.
Do we have to use your reagents together with your antibodies in our experiments?
At certain critical points, we strongly recommend using our reagents. Our experiences show that certain proteins in the whole cell lysate are at picogram and even femtogram levels. Detecting these proteins in Western blotting using regular ECL reagents is a seemingly impossible task. In this case, our custom PiQ (Cat#636) or FeQ (Cat#226) ECL reagents can detect picogram- and femtogram-level proteins, respectively. Second, we recommend you always use our FadeStop fluorescence mounting medium (Cat#270) or FadeStop fluorescence mounting medium with DAPI (Cat#272, to counterstain nuclei) in immunofluorescent staining. These antifade fluorescence mounting media have passed our testing and can preserve fluorescence after two months when stored at -20°C or after as many as twelve laser scans using a confocal microscope. Finally, we recommend using our IntactProteinTM Cell Lysis Kit (Cat#415) to extract whole cell lysates from cultured mammalian cells. This kit is particularly formulated to maintain the integrity as well as the signal moiety (such as phosphorylation) of all sizes of proteins. Since this kit avoids sonication, it protects proteins, particularly large-sized proteins (> 100 kDa), from fragmentation.
How should I store the antibodies?
Our antibodies are shipped to you via overnight carrier on ice pads. Once you receive the antibodies, store them at -20°C. It is a misconception that storing the antibodies at a lower temperature such as -80°C will increase their shelf-life. The antibodies are supplied in 50% glycerol; hence, the optimal temperature for storage is -20°C. Storing an antibody at any other temperature will shorten the antibody’s shelf life.
Can I use an antibody for applications other than those stated in your data sheet?
Yes, but we cannot guarantee that it will work. Our antibodies are only tested for use in Western blotting (WB), immunocytochemistry and immunofluorescence (IC-IF), and flow cytometry (F) applications. However, it is highly probable that these validated antibodies can be used in other applications.
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