IntactProtein™ Cell-Tissue Lysis Kit
Our unique and proprietary lysis buffer formulation (catalog #415) can not only perform all the protein extraction you are currently doing, but further serves as the ultimate solution for extracting large proteins and post-translationally modified (PTM) proteins. You do not need to add any inhibitors or phosphatases, or do any sonication. The kit comes in just two easy-to-use parts and works for mammalian cells and tissues (including adipose tissue). Like others in the past, you'll try this and never go back.
Summary of Key Features:
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Preserve the integrity of large proteins: Near-complete extraction of large proteins; no sonication required means no fragmentation
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Preserve protein PTMs: No loss of protein PTMs even without addition of inhibitors.
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All-in-one formula: No protease/other enzyme inhibitors or sonication required
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Ready-to-use protocol: Simply mix Reagents A & B; extraction takes as little as 15 min
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Wide-range of applications: Suitable for mammalian cells and tissues; equally effective for small and medium proteins
Competitive Analysis:
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Xu C, Mei Y, Yang R, Luo Q, Zhang J, Kou X, Hu J, Wang Y, Li Y, Chen R, Zhang Z, Yao Y, Sima J. Edaravone Dexborneol mitigates pathology in animal and cell culture models of Alzheimer's disease by inhibiting neuroinflammation and neuronal necroptosis. Cell Biosci. 2024 Apr 27;14(1):55. doi: 10.1186/s13578-024-01230-8. PMID: 38678262; PMCID: PMC11056062.
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