Proteinase K #664

Endoproteinase K 

Peptidase K 

Endopeptidase K 

For research use only  

Proteinase K is an active serine protease. It has nonspecific cleavage activity on a broad range of substrates including native and denatured proteins. Proteinase K is highly stable in the presence of denaturing agents, such as SDS and urea, and under a wide range of pH, temperature, and salt conditions. It is active between pH 4 and 12, and between 0 and 75°C.

 

Proteinase K is widely used in the field of molecular biology. Its applications include, but are not limited to, digesting proteins in the preparation of DNA or RNA, digesting and thus inactivating enzymes including nucleases and phosphatases to terminate reactions, retrieving antigens from formalin-fixed, paraffin-embedded specimens for immunohistochemical staining, and adding to tail lysis buffer for genotyping purposes.

Molecular Weight: 28.93 g/mol

Form: Lyophilized powder

Purity: >95% by native PAGE

Source: Recombinant proteinase K from tritirachium album, produced in yeast

Grade: Molecular biology

Units/mg protein: >40

One unit liberates 1 µmol of tyrosine per minute from casein at pH7.5 at 37°C. 

DNA/RNA: not detected 

DNase: not detected 

RNase: not detected 

Endonuclease: not detected 

Stable at -20°C for at least 1 year 

Do not store in frost-free freezer 

#664 Data Sheet