Highly Cross-Adsorbed Goat Anti Rabbit IgG (H+L) Ab, HRP  #202

Highly Cross-Adsorbed Goat Anti Rabbit IgG (H+L) Ab, HRP #202

$49.00Price

HRP-Conjugated Secondary Antibody

Secondary antibody
  • Species Reactivity

    Rabbit, highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera

  • Conjugate

    Horseradish peroxidase (HRP) 

  • Isotype

    Goat IgG 

  • Clonality

    Polyclonal 

  • Immunogen

    Rabbit IgG, heavy and light chains 

  • Tested Applications

    Western blotting (WB) 

    ELISA   

  • Disclaimer

    For research use only  

  • Storage

    Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C. 

  • Recommended Dilutions

    Western blotting (WB): 1:2,000

    ELISA: 1:20,000

  • Technical Note

    Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.  

  • Background

    HRP-conjugated goat anti-rabbit IgG secondary antibody is highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera. This secondary antibody is affinity-purified and is suitable for detecting rabbit primary antibodies used in WB, IHC and ELISA. First, a primary antibody of rabbit origin is bound to a target antigen. Following a brief wash, HRP-conjugated goat anti-rabbit secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors.

  • Product Features

    • Horseradish peroxidase conjugated
    • No cross-reactions with human, mouse, rat, bovine, or goat IgG
    • Validated with Western blotting and ELISA

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