Highly Cross-Adsorbed Goat Anti Rabbit IgG (H+L) Ab, HRP #202

Rabbit, highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera

Horseradish peroxidase (HRP) 

Goat IgG 

Polyclonal 

Rabbit IgG, heavy and light chains 

Western blotting (WB) 

ELISA   

For research use only  

Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C. 

Western blotting (WB): 1:2,000

ELISA: 1:20,000

Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.  

HRP-conjugated goat anti-rabbit IgG secondary antibody is highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera. This secondary antibody is affinity-purified and is suitable for detecting rabbit primary antibodies used in WB, IHC and ELISA. First, a primary antibody of rabbit origin is bound to a target antigen. Following a brief wash, HRP-conjugated goat anti-rabbit secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors.

• Horseradish peroxidase conjugated
• No cross-reactions with human, mouse, rat, bovine, or goat IgG
• Validated with Western blotting and ELISA

Highly Cross-Adsorbed Goat Anti Rabbit IgG (H+L) Ab, HRP  #202