Highly Cross-Adsorbed Goat Anti Rabbit IgG (H+L) Ab, HRP #202
HRP-Conjugated Secondary Antibody
Rabbit, highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera
Horseradish peroxidase (HRP)
Rabbit IgG, heavy and light chains
Western blotting (WB)
For research use only
Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C.
Western blotting (WB): 1:2,000
Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.
HRP-conjugated goat anti-rabbit IgG secondary antibody is highly cross-adsorbed against human, mouse, rat, bovine, and goat IgGs and sera. This secondary antibody is affinity-purified and is suitable for detecting rabbit primary antibodies used in WB, IHC and ELISA. First, a primary antibody of rabbit origin is bound to a target antigen. Following a brief wash, HRP-conjugated goat anti-rabbit secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors.
- Horseradish peroxidase conjugated
- No cross-reactions with human, mouse, rat, bovine, or goat IgG
- Validated with Western blotting and ELISA