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Goat Anti Rabbit Secondary Ab #201

$60.00Price

 

Secondary antibody
  • Species Reactivity

    Rabbit, cross-absorbed 

  • Conjugate

    Horseradish peroxidase (HRP) 

  • Isotype

    Goat IgG 

  • Clonality

    Polyclonal 

  • Immunogen

    Rabbit IgG, heavy and light chains 

  • Application

    Western blotting (WB) 

    Immunohistochemistry (IHC) 

    ELISA   

  • Disclaimer

    For research use only  

  • Storage

    Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C. 

  • Recommended Dilutions

    Western blotting (WB):  

           PiQ™ ECL Reagent: 1:1,000-1:5,000   

           FeQ™ ECL Reagent: 1:5,000-1:20,000  

    Immunohistochemistry (IHC): 1:200-1:1,000 

    ELISA: 1:100-1:200   

  • Caution

    Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since this product inhibits HRP enzyme activity.  

  • Technical Note

    HRP-conjugated goat anti-rabbit IgG secondary antibody is affinity-purified and is suitable to detect primary antibodies used in WB, IHC and ELISA. First, a primary antibody of rabbit origin is bound to a target antigen. After a brief wash, HRP-conjugated goat anti-rabbit IgG secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to generate detectable chemiluminescence or insoluble colors.

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