HRP-Conjugated Goat Anti Rabbit Secondary Ab #201
Species Reactivity
Rabbit, cross-absorbed
Conjugate
Horseradish peroxidase (HRP)
Isotype
Goat IgG
Clonality
Polyclonal
Immunogen
Rabbit IgG, heavy and light chains
Application
Western blotting (WB)
Immunohistochemistry (IHC)
ELISA
Disclaimer
For research use only
Storage
Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C.
Recommended Dilutions
Western blotting (WB):
PiQ™ ECL Reagent: 1:1,000-1:5,000
FeQ™ ECL Reagent: 1:5,000-1:20,000
Immunohistochemistry (IHC): 1:200-1:1,000
ELISA: 1:100-1:200
Caution
Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since this product inhibits HRP enzyme activity.
Technical Note
HRP-conjugated goat anti-rabbit IgG secondary antibody is affinity-purified and is suitable to detect primary antibodies used in WB, IHC and ELISA. First, a primary antibody of rabbit origin is bound to a target antigen. After a brief wash, HRP-conjugated goat anti-rabbit IgG secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to generate detectable chemiluminescence or insoluble colors.
Data Sheet