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PiQ™ ECL Substrate Kit #636

PiQ™ ECL Substrate Kit #636

  • Background

    Enhanced chemiluminescence (ECL) substrates are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in immunoassays. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by an X-ray film, digital imager or chemiluminescence plate reader.


    PiQ™ ECL Substrate Kit is formulated with proprietary enhancers that significantly increase the intensity and duration of light emitted. The PiQ™ ECL reagents are ultrasensitive and suitable for detecting proteins at the picogram level. When using these reagents in Western blotting, the recommended dilutions for primary and secondary antibodies are 1:5,000 and 1:50,000, respectively. We also tested this product in chemiluminescent ELISA, it outperformed other commercially available ECL reagents by generating much stronger signals with a relative low background.

  • Storage

    Stable at 4°C for at least 1 year

  • Experimental Protocol

    • Let the ECL reagents warm up at room temperature for approximately 30 min prior to use.
    • Combine Reagents A and B at a ratio of 1:1. Vortex to mix.
    • Apply the mixture to the Western blots or microplate wells.
    • Immediately capture signals with a digital imager or chemiluminescence plate reader.
  • Application Tested

    Western blotting

    Chemiluminescent ELISA

  • Product Features

    • Superior performance in generating extremely intense signals
    • Suitable for detecting low-abundance analytes
    • Optimized for Western blotting and chemiluminescent ELISA
  • Data Sheet




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