DryMicro RT-LAMP Master Mix
For RT-LAMP and Multiplex RT-LAMP
Simple solution: All-in-one solution with all the components included, except primer set(s)
Easy to store: The product can be stored at room temperature for over one year and a half without loss of quality
Improved stability: Freeze-drying increases long-term stability and consistency in product performance
Enhanced flexibility: It can be used with 1) visible violet-to-blue endpoint detection; 2) real-time fluorescent detection at FAM fluorescent channel; and 3) single or multiplex detection with lateral flow test strips. For lateral flow detection, two primers can be labeled with biotin and DIG, respectively, and/or biotin and FAM, respectively.
Detection without RNA extraction: One of the remarkable features of this product is that you do not have to extract RNA before performing LAMP. Simply lysing the pathogen in our proprietary IntactViral™ buffer (#IV100), add the lysate directly to the lyophilized microsphere, and you can run the LAMP the same way you use purified RNA.
One and a half years if the package is not open.
Reverse transcriptase, Bst DNA polymerase, MgCl2, dNTPs, optimized PCR buffer, an endpoint reading dye, a fluorescent dye which can be detected at FAM fluorescent channel, and a proprietary mixture of lyoprotectants.
Store at room temperature.
Quick Reference Guide
- For purified viral RNA: Add 27 µL of nuclease-free water to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
- For heat-inactivated viruses or human specimens: Add 27 µL of IntactViral™ buffer (#IV100) containing heat-inactivated viruses or human specimens to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
- Add LAMP primer set(s) to the rehydrated master mix, bring the total volume of the mix to 30 µL with nuclease-free water. Vortex for 3 seconds and centrifuge briefly to make sure all solutions are collected at the bottom of the tube.
- For endpoint detections, including visual detection of color change and lateral strip detection: Load the tube onto a heat block or thermocycler, and run the LAMP reaction at 65 ºC for 45-60 min. For real-time fluorescent detection at FAM channel: Run the LAMP reaction at 65 ºC for 45-60 min. Fluorescent signals can be captured every 1 min or at the desired time interval according to the needs of the experiment.
Since its introduction in 2000, loop-mediated isothermal amplification (LAMP) has been widely used in to detect the DNA of humans, animals, and plants. Today, LAMP has been modified to detect nucleic acids in real-time (real-time LAMP), with more than one target (multiplex LAMP or M-LAMP), and RNA (RT-LAMP). Though attractive in terms o