DryMicro PCR Master Mix #DMD101

Simple workflows: All-in-one solution with all the components included, except primers and probe(s)

Easy to store: The product can be stored at room temperature for over a year and a half without loss of quality

Enhanced Stability: Freeze-drying increases long-term stability and consistency in product performance

Enhanced flexibility: It can be used with an end point as well as real-time fluorescent detection

Detection without DNA extraction: One of the remarkable features of this product is that you do not have to extract DNA before performing a PCR. Simply lysing the pathogen in our proprietary ExtractFree™ buffer (#IV100), add the lysate directly to the lyophilized microsphere, and you can run the real-time PCR the same way you use purified DNA.

One and a half years if the package is not open.

Thermostable DNA polymerase, MgCl2, dNTPs, optimized PCR buffer, and a proprietary mixture of lyoprotectants. Not included: fluorescent dye. If you run a qPCR without using a probe, you need to add SYBR Green or purchase our fluorescent dye (Cat# FL110) which can be detected in the FAM fluorescent channel.

Store at room temperature.

• Add 17 µL of nuclease-free water to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
• Alternatively, add  17 µL ofExtractFree™ buffer (#IV100) with the viruses to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
• Load the tube onto a PCR or real-time PCR detection system. If you use a 96- or 384-well plate, you can transfer the mix into the designated wells.
• Perform PCR according to the following program:

                 Step 1: 95ºC, 2 minutes; 1 cycle

                 Step 2: 95 ºC, 5 seconds, then 60 ºC, 10 seconds; 45 cycles.

DMD101

PCR and real-time PCR (qPCR) are standard laboratory and diagnostic techniques for the detection of DNA. Most commercial master mixes come in the form of solutions, requiring a cold chain for transportation and freezers for storage. To facilitate transport and storage, we optimize the master mix and lyophilize it in the form of microspheres. With the addition of solution, the lyophilized microspheres dissolve instantly without sacrificing its original qualities. Not only does the rehydrated master mix match the performance of established brands, but it can also be used for the detection of pathogens without the DNA extraction step, thus greatly reducing time and costs.