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DryMicro LAMP Master Mix

DryMicro LAMP Master Mix

$159.00Price

For LAMP and Multiplex LAMP

  • Key Features

    Simple solution: All-in-one solution with all the components included, except primer set(s)

    Easy to store: The product can be stored at room temperature for over one year and a half without loss of quality

    Improved stability: Freeze-drying increases long-term stability and consistency in product performance

    Enhanced flexibility: It can be used with 1) visible violet-to-blue endpoint detection; 2) real-time fluorescent detection at FAM fluorescent channel; and 3) single or multiplex detection with lateral flow test strips. For lateral flow detection, two primers can be labeled with biotin and DIG, respectively, and/or biotin and FAM, respectively.

    Detection without DNA extraction: One of the remarkable features of this product is that you do not have to extract DNA before performing LAMP. Simply lysing the pathogen in our proprietary IntactViral™ buffer (#IV100), add the lysate directly to the lyophilized microsphere, and you can run the LAMP the same way you use purified DNA.

  • Expiration Date

    One and a half years if the package is not open.

  • Components

    Bst DNA polymerase, MgCl2, dNTPs, optimized PCR buffer, an endpoint reading dye, a fluorescent dye which can be detected at FAM fluorescent channel, and a proprietary mixture of lyoprotectants.

  • Storage Condition

    Store at room temperature.

  • Quick Reference Guide

    • For purified viral DNA: Add 27 µL of nuclease-free water to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
    • For heat-inactivated viruses or human specimens: Add 27 µL of IntactViral™ buffer (#IV100) containing heat-inactivated viruses or human specimens to a tube containing 1 lyophilized microsphere, vortex for 5 seconds and spin down the solution using a micro centrifuge. The lyophilized powder shall dissolve immediately.
    • Add LAMP primer set(s) to the rehydrated master mix, bring the total volume of the mix to 30 µL with nuclease-free water. Vortex for 3 seconds and centrifuge briefly to make