Anti-S-Tag Mouse Monoclonal Ab#T103

S-tag; S-Peptide epitope tag; S-peptide

Adding a polypeptide, small protein, or enzyme to a gene of interest, also referred to as protein tagging, is a powerful and versatile technique widely used in biochemistry and molecular biology. In practice, an epitope tag is cloned in-frame relative to the gene of interest, either at the amino (N) or carboxy (C) terminus of the gene. As a result, a fusion protein is synthesized with the peptide tag.

Three-Letter Sequence: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser

One-Letter Sequence: KETAAAKFERQHMDS

Clonality: Mouse monoclonal antibody

Clone ID: 21GB196

Species Reactivity: All

Applications Tested: Western blotting (WB)

Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide.  

Store at -20 °C for one year.

Western blotting (WB): 1:1,000

• Protein purification
• Detection of the fusion protein, particularly useful when there is no specific antibody to the protein of interest
• Subcellular visualization of the fusion protein
• Western blotting (WB), immunocytochemistry (IC), and (co-)immunoprecipitation (IP)

#T103 S-Tag DS