Ultrasensitive Streptavidin-Polymer-HRP  #505

Ultrasensitive Streptavidin-Polymer-HRP #505

$89.00Price

HRP and streptavidin linked to a polymer backbone

Secondary antibody
  • Reactivity

    Biotinylated proteins, DNA, RNA, and other macromolecules

  • Conjugate

    Horseradish peroxidase (HRP) and streptavidin are conjugated to a hydrophilic polymer backbone.

  • Tested Applications

    ELISA   

  • Disclaimer

    For research use only  

  • Storage

    Supplied in PBS, pH 7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C.

  • Recommended Dilutions

    ELISA: 1:10,000

  • Technical Note

    Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.  

  • Background

    Biotinylated proteins, including primary and secondary antibodies, are widely used for protein purification and immunoassays. Due to its strong binding affinity to streptavidin, biotinylated proteins are often used with streptavidin conjugates such as streptavidin-fluorescent dyes and streptavidin-HRP to amply signal and increase detection sensitivity. Streptavidin-polymer-HRP combines the specificity of streptavidin-biotin interaction with signal amplification via conjugation of multiple HRPs to a hydrophilic polymer backbone. Applications of this highly sensitive biotinylated protein assay include Western blotting, ELISA, immunoprecipitation, immunocytochemistry, and immunohistochemistry.

  • Product Features

    • Highly sensitive, suitable for detection of low-abundance macromolecules
    • Extremely low background compared to other amplification methods
    • Broad range of applications such as WB, ELISA, IP, IC, and IHC

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