Ultrasensitive Streptavidin-Polymer-HRP #505
HRP and streptavidin linked to a polymer backbone
Biotinylated proteins, DNA, RNA, and other macromolecules
Horseradish peroxidase (HRP) and streptavidin are conjugated to a hydrophilic polymer backbone.
For research use only
Supplied in PBS, pH 7.2 and 50% glycerol. This product is stable for 2 years when stored at -20°C.
Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.
Biotinylated proteins, including primary and secondary antibodies, are widely used for protein purification and immunoassays. Due to its strong binding affinity to streptavidin, biotinylated proteins are often used with streptavidin conjugates such as streptavidin-fluorescent dyes and streptavidin-HRP to amply signal and increase detection sensitivity. Streptavidin-polymer-HRP combines the specificity of streptavidin-biotin interaction with signal amplification via conjugation of multiple HRPs to a hydrophilic polymer backbone. Applications of this highly sensitive biotinylated protein assay include Western blotting, ELISA, immunoprecipitation, immunocytochemistry, and immunohistochemistry.
- Highly sensitive, suitable for detection of low-abundance macromolecules
- Extremely low background compared to other amplification methods
- Broad range of applications such as WB, ELISA, IP, IC, and IHC