top of page
Product Page: Stores_Product_Widget
ShQ™ ECL Substrate Kit #707

ShQ™ ECL Substrate Kit #707


ECL for Chemiluminescent ELISA

  • Background

    Enhanced chemiluminescence (ECL) substrates are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in immunoassays. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by an X-ray film, digital imager or chemiluminescence plate reader. ShQ™ ECL Substrate Kit is proprietarily formulated for chemiluminescent ELISA test. Compared to ultrasensitive PiQ™ ECL, ShQ™ ECL Substrate Kit delivers superior performance by generating an extremely low background.

  • Storage

    Stable at 4°C for at least 1 year

  • Experimental Protocol

    • Let the ECL reagents warm up at room temperature for approximately 30 min prior to use.
    • Combine Reagents A and B at a ratio of 1:1. Vortex to mix.
    • Apply the mixture to the microplate wells.
    • Immediately read the relative light unit (RLU) of the chemiluminescence signals using a chemiluminescence plate reader.
  • Application Tested

    Chemiluminescent ELISA

  • Recommended Antibody Dilution

    Primary antibody: 1: 5,000 - 1:10,000

    Secondary antibody: 1: 10,000 - 1:20,000

  • Kit Component

    Equal volume of Reagents A and B

  • Product Features

    • Superior performance with an ultra-low background
    • Suitable for detecting highly abundance analytes
    • Ready-to-use kit with easy-to-follow instructions