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HRP-Conjugated Goat Anti-Mouse Secondary Ab


Species Reactivity: Mouse, cross-absorbed

ConjugateHorseradish peroxidase (HRP)

Isotype: Goat IgG

Clonality: Polyclonal

Immunogen: Mouse IgG, heavy and light chains

Application: Western blotting (WB); Immunohistochemistry (IHC); ELISA  

Disclaimer: For research use only.

Storage: Supplied in PBS, pH7.2 and 50% glycerol. This product is stable for 2 years when stored at -20oC.

Recommended Dilutions:

Western blotting (WB):

   PiQTM ECL Reagent: 1:1,000-1:5,000 

   FeQTM ECL Reagent: 1:5,000-1:20,000

Immunohistochemistry (IHC): 1:200-1:1,000

ELISA: 1:100-1:200 

Description: HRP-conjugated goat anti-mouse IgG secondary antibody is affinity-purified and is suitable to detect primary antibodies used in WB, IHC and ELISA. First, a primary antibody of mouse origin is bound to a target antigen. After a brief wash, HRP-conjugated goat anti-mouse IgG secondary antibody is applied to form a complex with the primary antibody. After a brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to generate detectable chemiluminescence or insoluble color.     

Caution: Do not use sodium azide (NaN3) as a

bacteriostatic preservative in antibody dilution buffer, since this product inhibits HRP enzyme activity.

2nd antibody