Goat Anti Rabbit IgG (H+L), Polymer-HRP  #303

Goat Anti Rabbit IgG (H+L), Polymer-HRP #303

$69.00Price

Polymer-HRP-Conjugated Secondary Antibody

Secondary antibody
  • Species Reactivity

    Rabbit

  • Conjugate

    Polymer-Horseradish peroxidase (HRP) 

  • Isotype

    Goat IgG 

  • Clonality

    Polyclonal 

  • Immunogen

    Rabbit IgG, heavy and light chains 

  • Tested Applications

    ELISA   

  • Disclaimer

    For research use only  

  • Storage

    Supplied in 100x concentrated solution. Store at 4C.

  • Recommended Dilutions

    ELISA: 1:100-1:300

  • Technical Note

    Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.  

  • Background

    This secondary antibody is affinity-purified and is suitable for detecting rabbit IgG used in ELISA. Polymer-HRP conjugation increases sensitivity and minimizes background detection. First, a primary antibody of rabbit origin is bound to a target antigen. Following a brief wash, Polymer-HRP-conjugated goat anti-rabbit IgG secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors.

  • Product Features

    • Conjugated to a polymer backbone linked to numerous HRP enzymes
    • Increases assay sensitivity by up to 50-fold as compared to single HRP conjugation
    • Suitable for amplifying low-abundance protein and low-affinity antigen-antibody binding signals

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Orders

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