Goat Anti Rabbit IgG (H+L), Polymer-HRP #303
Polymer-HRP-Conjugated Secondary Antibody
Polymer-Horseradish peroxidase (HRP)
Rabbit IgG, heavy and light chains
For research use only
Supplied in 100x concentrated solution. Store at 4C.
Do not use sodium azide (NaN3) as a bacteriostatic preservative in antibody dilution buffer, since NaN3 inhibits HRP enzyme activity.
This secondary antibody is affinity-purified and is suitable for detecting rabbit IgG used in ELISA. Polymer-HRP conjugation increases sensitivity and minimizes background detection. First, a primary antibody of rabbit origin is bound to a target antigen. Following a brief wash, Polymer-HRP-conjugated goat anti-rabbit IgG secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors.
- Conjugated to a polymer backbone linked to numerous HRP enzymes
- Increases assay sensitivity by up to 50-fold as compared to single HRP conjugation
- Suitable for amplifying low-abundance protein and low-affinity antigen-antibody binding signals