GenuIN® 4x IP Viral Lysis Buffer #VL202

GenuIN® 4x IP Viral Lysis Buffer #VL202

$79.00Price

Non-Denaturing Lysis Buffer Used for IP

Reagents
  • Background

    Complete lysis of SARS-CoV-2 and host cells in human specimens is the first step in downstream immunoassays. The GenuIN® 4x IP Viral Lysis Buffer is proprietarily formulated to lyse biospecimens of patients with COVID-19 or SARS-CoV-2 coronaviruses from cultured cells. This non-denaturing buffer contains a special formula for complete lysis of viral and cellular proteins while having little interference with antigen-antibody binding. So it is an ideal option for immunoprecipitation.

  • Storage

    Stable at 4 ℃ for at least 1 year

  • Experimental Protocol

    • Mix 1 part of GenuIN® 4x IP Viral Lysis Buffer with 3 parts of distilled H2O or biospecimens to make 1x Viral Lysis Buffer.
    • Add a protease or phosphatase inhibitor cocktail (not provided) to prevent proteolysis and phosphorylation loss.
    • Lyse the cells or specimens on ice for 20 min with constant agitation or vortexing.
    • Store lysates at -20°C or use them immediately for downstream applications.
  • Tested Application

    Immunoprecipitation (IP)

  • Features of the Product

    • Tested and optimized for immunoprecipitation (IP) application
    • Ready-to-use solution
    • Effective for the extraction of nuclear, cytosolic and membrane proteins

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