FeQ™ ECL Substrate Kit #226

Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers.

FeQ™ ECL Substrate Kit is formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light. FeQ™ ECL reagents are ultrasensitive and suitable to detect proteins at femtogram level. When using these reagents, the recommended dilutions for primary and secondary antibodies are 1:10,000 and 1:100,000, respectively. Compared to other commonly used ECL reagents, ours exhibit superior performance by generating extremely intense signal with low background and require ten-fold less primary and secondary antibodies.

Stable at 4°C for at least 1 year

1. Allow the ECL reagents to equilibrate to room temperature approximately 30 min before use.
2. Wash the membrane thoroughly to reduce non-specific signals.
3. Mix Reagent A and B at a 1:1 ratio. Vortex to mix.
4. Immerse the membrane in the mixed solution for 2–3 min.
5. Drain the excess solution using a paper towel and wrap the membrane in plastic wrap or a clear plastic folder.
6. Detect the chemiluminescence using X-ray film or a digital imager.

#226 Data Sheet