GenuIN® Cov2 Enrichment Kit #VKN1

Accurate detection of SARS-CoV-2 is difficult, particularly when viral levels are low. First, no tests, whether RT-PCR or immunoassay, are 100% accurate; consequently, false negative or positive results are unavoidable (Shuren and Stenzel, 2020). False negative outcomes will increase the risk of viral spread, while false positive outcomes will increase the burden on healthcare providers and unnecessary quarantine (Shuren and Stenzel, 2020). Second, the virial load of various types of clinical specimens can vary considerably. Clinical studies indicate that lower respiratory tract specimens tend to have a higher viral load (Hu et al., 2020; Wang et al., 2020). Specifically, the bronchoalveolar lavage fluid specimens contain the highest vial load, followed by sputum, nasal swabs, fibrobronchoscope brush biopsy, pharyngeal swabs, feces, and blood (Wang et al., 2020). Finally, the presence of SARS-CoV-2 in water and wastewater transmitted from patients’ feces and masks has raised significant environmental concerns (Tran et al., 2020). However, it is technically challenging to detect SARS-CoV-2 at a very low level.

 

One way to increase the sensitivity and accuracy of SARS-CoV-2 detection is to enrich the coronaviruses from biospecimens or samples. To this end, we developed the GenuIN® CoV2 Enrichment Kit to concentrate SARS-CoV-2 nucleocapsid proteins in solutions with magnetic beads. Enriched N proteins can be used for subsequent applications such as immunoprecipitation, ELISA, lateral flow immunoassay, and proteomic research.

 

Hu, B., Guo, H., Zhou, P., and Shi, Z.L. (2020). Characteristics of SARS-CoV-2 and COVID-19. Nat Rev Microbiol.

Shuren, J., and Stenzel, T. (2020). Covid-19 Molecular Diagnostic Testing - Lessons Learned. N Engl J Med 383, e97.

Tran, H.N., Le, G.T., Nguyen, D.T., Juang, R.S., Rinklebe, J., Bhatnagar, A., Lima, E.C., Iqbal, H.M.N., Sarmah, A.K., and Chao, H.P. (2020). SARS-CoV-2 coronavirus in water and wastewater: A critical review about presence and concern. Environ Res, 110265.

Wang, W., Xu, Y., Gao, R., Lu, R., Han, K., Wu, G., and Tan, W. (2020). Detection of SARS-CoV-2 in Different Types of Clinical Specimens. JAMA 323, 1843-1844.

• Reay-to-use kit including required components
• Detailed instructions are simple to understand and follow
• Suitable to enrich SARS-CoV-2 at very low levels

For research use only.

GenuIN® Cov2 Enrichment Kit #VKN1