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Anti-V5-Tag Mouse Monoclonal Ab#T105

Anti-V5-Tag Mouse Monoclonal Ab#T105



  • Aliases

    V5-Tag; V5 Epitope Tag

  • Background

    Adding a polypeptide, small protein, or enzyme to a gene of interest, also referred to as protein tagging, is a powerful and versatile technique widely used in biochemistry and molecular biology. In practice, an epitope tag is cloned in-frame relative to the gene of interest, either at the amino (N) or carboxy (C) terminus of the gene. As a result, a fusion protein is synthesized with the peptide tag.

  • Amino Acid Sequence

    Three-Letter Sequence: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

    One-Letter Sequence: GKPIPNPLLGLDST

  • Antibody Description

    Clonality: Mouse monoclonal antibody

    Clone ID: 21GB198

    Species Reactivity: All

    Applications Tested: Western blotting (WB)

  • Storage Buffer

    Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide.  

  • Storage Instruction

    Store at -20 °C for one year.

  • Recommended Dilution

    Western blotting (WB): 1:1,000


  • Applications

    • Protein purification
    • Detection of the fusion protein, particularly useful when there is no specific antibody to the protein of interest
    • Subcellular visualization of the fusion protein
    • Western blotting (WB), immunocytochemistry (IC), and (co-)immunoprecipitation
  • Data Sheet




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