Anti-BrdU Mouse Monoclonal Ab#61627

Anti-BrdU Mouse Monoclonal Ab#61627


Application Validated

  • Aliases

    Bromodeoxyuridine (BrdU)

  • Application Information

    Molecular Weight:       N/A

    Clonality:                       Mouse monoclonal antibody

    Clone ID:                     20GB140

    Species Reactivity:        All

    Applications Tested:   Immunofluorescence (IF)

  • Cellular Localization


  • Immunogen


  • Isotype

    Mouse IgG  

  • Storage Buffer

    Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide.  

  • Storage Instruction

    Product should be stored at –20°C

  • Recommended Dilution

    Immunofluorescence (IF): 1:500


  • Protocols

    For general and antibody-specific protocols, please visit our website. Read all instructions carefully before using this product.    

  • Data Sheet

    Available upon request

  • Technical Note

    IF protocol

    1. Discard culture medium, wash the cells with ice-cold PBS 3 X 5 min.
    2. Fix cells with ice-cold 70% ethanol for 5 min at room temperature.
    3. Wash the cells with ice-cold PBS 3 X 5 min.
    4. Incubate cells with 1.5 M HCl for 30 min at room temperature.
    5. Wash the cells with ice-cold PBS 3 X 5 min.
    6. Proceed with blocking and immunostaining.






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